Phosphotransferase

Phosphotransferase (PT) are enzymes which catalyze phosphorylation reactions. The acceptor group can be alcohol, carboxy, nitrogenous, phosphate or a pair ofd groups. Aminoglycoside PT (AGPT) inactivate aminoglycoside antibiotics by phosphorylation. The phosphoenolpyruvate-protein PT (PEP) catalyzes the phosphorylation of protein histidine residues. See Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System for some details.

3D structures of phosphotransferase
3our – PT IIA + pyruvate decarboxylase – Vibrio vulnificus 2l5h, 2kx9 - EcPT I - Escherichia coli – NMR 2xdf - EcPT I+ phosphocarrier protein HPR – NMR 3oxp – PT II – Yersinia pestis 3ci6 – PT GAF domain – Acinetobacter 2ooc – PT histidine – Caulobacter crescentus 3ipj – PT IIABC – Clostridium difficile 2f9h - PT IIA – Enterococcus faecalis

Aminoglycoside phosphotransferase
3ovc - EcAGPT IA 3r6z - EcAGPT IB 3q2m, 3i1a – LpAGPT IA – Legionella pneumophila 1nd4 – KpAGPT IIA – Klebsiella pneumoniae 1qhn – SvAGPT – Streptomyces venezuelae 3sgc, 3r7z, 3n4t, 3n4u, 3n4v - EncAGPT ID – Enterococcus casseliflavus 1j7i - AGPT IIIA – Enterococcus faecalis

Aminoglycoside phosphotransferase complex with nucleotide
3r70 - EcAGPT IB + ADP 3r78 - EcAGPT IB + ATP 1qhx - SvAGPT + ATP<BR /> 3i0q - LpAGPT IA + AMP<BR /> 1j7l - AGPT IIIA + ADP – Enterococcus faecalis<BR /> 1j7u - AGPT IIIA + AMP-PNP – Enterococcus faecalis

===Aminoglycoside phosphotransferase complex with antibiotic ===

3hav, 3ham - EfAGPT + ATP + antibiotic – Enterococcus faecium<BR /> 3h8p - EfAGPT + AMP-PNP + antibiotic

2b0q, 1l8t - EfAGPT IIIA + ADP + antibiotic

3r80, 3r81 - EcAGPT ID + antibiotic

1grq, 1grr, 1qhs - SvAGPT + antibiotic

1qhy - SvAGPT + ATPγ + antibiotic

3sg8, 3sg9 - EncAGPT ID + antibiotic

3i0o - LpAGPT IA + ADP + antibiotic

Aminoglycoside phosphotransferase complex with inhibitor
3q2j – AGPT IIIA + inhibitor – Enterococcus faecalis<BR /> 2bkk - EfAGPT IIIA + inhibitor

Phosphoenolpyruvate-protein phosphotransferase
2xz7 – TtPEP PEP-binding domain + phosphoenolpyruvate – Thermoanaerobacter tengcongensis<BR /> 2xz9 - TtPEP PEP-binding domain + pyruvate<BR /> 3nbm – PEP lactose-specific IIBC – Streptococcus pneumoniae<BR /> 2l2q - PEP cellbiose-specific IIB – Borrelia burgdorferi – NMR<BR /> 3k1s - PEP cellbiose-specific IIA – Bacillus anthracis<BR /> 2kyr – EcPEP fructose-specific IIB<BR /> 2r4q - BsPEP fructose-specific IIABC – Bacillus subtilis<BR /> 2r48 - BsPEP fructose-specific IIB<BR /> 1vsq, 2jzh, 2jzn, 2jzo - EcPEP mannose-specific IIA - NMR<BR /> 3bp3 - EcPEP glucose-specific IIB<BR /> 1o53 - EcPEP glucose-specific IIA N terminal – NMR<BR /> 1ggr - EcPEP glucose-specific IIA + phosphocarrier protein HPR – NMR<BR /> 3bp8 - EcPEP glucose-specific IIB + NAGC-like transcriptional regulator<BR /> 3eye - EcPEP acetylgalactosamine-specific IIB<BR /> 2oq3, 1vrv - EcPEP mannitol-specific IIA<BR /> 1vkr - EcPEP mannitol-specific IIABC – NMR<BR /> 1j6t - EcPEP mannitol-specific IIABC + phosphocarrier protein HPR – NMR<BR /> 2few - EcPEP mannitol-specific IIA (mutant) <BR /> 1wcr - EcPEP diacetylchitobiose-specific IIA (mutant) – NMR<BR /> 1h9c - EcPEP diacetylchitobiose-specific IIB – NMR<BR /> 3ipr – EfPEP gluconate-specific IIA<BR /> 3bed - EfPEP mannose-specific IIA<BR /> 1nrz - KpPEP sorbose-specific IIB